The wide range of REMBRANDT® products comprise complete kits for the detection of pan Human papilloma virus (HPV screening), HPV typing, Cytomegalo virus (CMV), Herpes simplex virus (HSV) 1/2, Epstein-Barr virus (EBV), Epstein-Barr encoded small mRNA's (EBER) and kappa/lambda light chain mRNA's. All probes are available with either biotin or digoxigenin as choice of probe label and are available with either an alkaline phosphatase (AP)/NBT-BCIP or horseradish-peroxidase (HRP)/AEC enzyme, substrate, chromogen combination, for your personal choice of detection.
|
Probe label
|
BIOTIN
|
DIGOXIGENIN
|
||
| Antibody conjugate Substrate Chromogen |
Anti BIO-AP
NBT/BCIP |
Anti BIO-HRP
Peroxide/AEC |
Anti DIG-AP
NBT/BCIP |
Anti DIG-HRP
Peroxide/AEC |
| REMBRANDT® DNA in-situ hybridisation (DISH) |
product code
|
product code
|
product code
|
product code
|
| Universal DISH kit |
A001K.0105
|
A001K.0101
|
A001K.9905
|
A001K.9901
|
| Human Papilloma virus screening |
A100K.0105
|
A100K.0101
|
A100K.9905
|
A100K.9901
|
| Human Papilloma virus typing |
A103K.0105
|
A103K.0101
|
A103K.9905
|
A103K.9901
|
| Human cytomegalo virus |
A200K.0105
|
A200K.0101
|
A200K.9905
|
A200K.9901
|
| Epstein-Barr virus |
A300K.0105
|
A300K.0101
|
A300K.9905
|
A300K.9901
|
| Herpes simplex virus 1/2 |
A400K.0105
|
A400K.0101
|
A400K.9905
|
A400K.9901
|
| REMBRANDT® RNA in-situ hybridisation (RISH) |
product code
|
product code
|
product code
|
product code
|
| Universal RISH kit |
A000K.0105
|
A000K.0101
|
A000K.9905
|
A000K.9901
|
| Epstein-Barr early mRNAs (EBER) |
A500K.0105
|
A500K.0101
|
A500K.9905
|
A500K.9901
|
| Kappa/Lambda light chain mRNAs |
C600K.0105
|
C600K.0101
|
C600K.9905
|
C600K.9901
|
Universal in situ hybridisation and detection
kits
The complete REMBRANDT® Universal ISH and Detection kits
contain all reagents and materials for the preparation, pre-treatment and detection
of labelled DNA or oligonucleotides by using the DNA (DISH)
or RNA (RISH) in situ hybridisation technique. Positive and
negative control probes are also included in these kits as positive and negative
controls for the in situ hybridisation and detection procedure. The kits are
offered for the detection of either biotin or digoxigenin labelled probes and
are available with either an alkaline phosphatase (AP)/NBT-BCIP or horseradish-peroxidase
(HRP)/AEC enzyme, substrate, chromogen combination, for your personal choice
of detection.
| BIO-AP BIO-HRP DIG-AP DIG-HRP |
: Biotin labelled probes combined with AP and
NBT/BCIP detection : Biotin labelled probes combined with HRP and AEC detection : Digoxigenin labelled probes combined with AP and NBT/BCIP detection : Digoxigenin labelled probes combined with HRP and AEC detection |
The reagents in the kit have been optimised for use with probes labelled by the Universal Linkage System (ULS®). This labelling method results in probes that are unique in performance and reproducibility.
REMBRANDT® Universal ISH and Detection kits are therefore ideally suited for use in combination with one of our broad range of compatible labelled probes. Moreover, also home-made probes are perfectly suited for the listed Rembrandt® detection systems.
Furthermore, the DISH and especially RISH Detection kits can also be applied in routine immunohistochemistry for the detection of either biotin or digoxigenin labelled antibodies, thus serving different applications and cost saving.
IMPORTANT NOTICE
- The Universal DISH and Detection kits are supplied with PanWash differentiation
reagent R013R.0000 for DNA probes with a GC% content < 50% (e.g. HPV).
- For the detection of DNA with a GC% content >50% (e.g. CMV, HSV and EBV),
the appropriate PanWash differentiation reagent R014R.0000 is offered separately
as listed in the Building Block section.
- The Rembrandt complete kits contain their specific PanWash differentiation
reagent; either R013R.0000 or R014R.0000.
- The single DNA probe products are packaged with their specific differentiation
reagent; either R013R.0000 or R014R.0000.
- The Universal RISH and Detection kits do not contain
such PanWash differentiation reagents because the nature of oligonucleotides
make such differentiation reagents obsolete.